Challenges and opportunities for siRNA-based cancer treatment

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• 作者：Tao Wang^a ; ^b ; Sarah Shigdar^b ; Hadi Al Shamaileh^b ; Michael P. Gantier^c ; ^d ; Wang Yin^b ; Dongxi Xiang^b ; Lan Wang^a ; Shu-Feng Zhou^e ; Yingchun Hou^f ; Peng Wang^a ; Weihong Zhang^a ; Chunwen Pu^g ; ^{dahaixiaowen@163.com} ; Wei Duan^b ; ^{wduan@deakin.edu.au}
• 关键词：RNA interference ; Tumour ; Nanoparticle ; EPR effect ; Endosome escape ; Off-target
• 刊名：Cancer Letters
• 出版年：2017
• 出版时间：28 February 2017
• 年：2017
• 卷：387
• 期：Complete
• 页码：77-83
• 全文大小：740 K
• 卷排序：387

文摘

Distinct rules for chemical modification of siRNA and antisense oligonucleotide. Optimised research model leads to predictable bio-distribution pattern of siRNAs. Characterisation of endosomal escape aids future siRNA-based cancer treatment. Gene target selection – cancer stem cell critical genes and multiple gene targeting. Appropriate research controls and 5′RACE assay ensure minimum off-target effect.