Theoretical and experimental study of enzyme kinetics in a microreactor system with surface-immobilized biocatalyst

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• 作者：Nata&scaron ; a Miložič^a ; Martin Lubej^a ; Mitja Lakner^b ; Polona Žnidar&scaron ; ič-Plazl^a ; Igor Plazl^a ; ^{igor.plazl@fkkt.uni-lj.si}
• 关键词：Microreactor ; Enzyme immobilization ; Surface-enzyme kinetics ; Transamination ; Mathematical modelling
• 刊名：Chemical Engineering Journal
• 出版年：2017
• 出版时间：1 April 2017
• 年：2017
• 卷：313
• 期：Complete
• 页码：374-381
• 全文大小：1156 K
• 卷排序：313

文摘

Continuous transamination in a microreactor with ω-transaminase was modeled. Zbasic2-tagged enzyme was immobilized on the inner surface based on ionic interactions. Surface reaction kinetics based on a ping-pong bi-bi mechanism was applied. Based on time-scale analysis, a simplified 1D model was developed and verified. Model enabled enzyme concentration estimation and microreactor performance forecast.