3D engineered In vitro hepatospheroids for studying drug toxicity and metabolism

详细信息    查看全文

• 作者：Swati Chitrangi^a ; Prabha Nair^b ; Aparna Khanna^a ; ^{aparna.khanna@nmims.edu}
• 关键词：3D ; Three-dimensional ; 2D ; Two-dimensional ; CYP ; cytochrome P450 ; GEVAC ; Gelatin-Vinyl Acetate ; MSCs ; Mesenchymal stem cells ; hUC-MSCs ; human umbilical cord derived-mesenchymal stem cells ; HLCs ; Hepatocyte-like cells ; DMEM-LG ; Dulbecco's Modified Eagle's-low glucose ; FBS ; Fetal Bovine Serum ; CD ; Cluster of Differentiation ; AST ; aspartate aminotransferase ; ALT ; alanine aminotransferase ; HR-LC/MS ; high resolution-liquid chromatography mass spectrometry ; m/z ; mass-to-charge ratio ; 7-AAD ; 7-amino ac
• 刊名：Toxicology in Vitro
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：38
• 期：Complete
• 页码：8-18
• 全文大小：2196 K
• 卷排序：38

文摘

Established stem cell-derived hepatospheroids in vitro drug screening model Comparison of 2D HLCs and 3D hepatospheroids for drug metabolism Effect of toxicity (ethanol, acetaminophen) on the functionality of hepatocytes ROS production indicated biomimetic toxic response. In vitro drug metabolite profiling indicated the presence of active CYP enzymes.