Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one duplex (pat/bar) real-time PCR methods for GMO detection

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• 作者：F. Debode^a ; ^{f.debode@cra.wallonie.be} ; I. Huber^b ; R. Macarthur^c ; P.E. Rischitor^d ; M. Mazzara^d ; V. Herau^f ; D. Sebah^b ; D. Dobnik^g ; S. Broeders^e ; N.H. Roosens^e ; U. Busch^b ; G. Berben^a ; D. Morisset^g ; J. Zel^g
• 关键词：GMO ; Validation ; Real-time PCR ; Screening ; Inter-laboratory study
• 刊名：Food Control
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：73
• 期：part_PB
• 页码：452-461
• 全文大小：844 K
• 卷排序：73

文摘

A new endogenous target for the detection of pea was developed. Singleplex and multiplex real-time PCR screening methods for GMO detection were validated. In-house validation, method transferability and inter-laboratory validation are described. Statistical approaches for the validation of qualitative methods are presented.