Anti-inflammatory effects of methanol extracts of the root of Lilium lancifolium on LPS-stimulated Raw264.7 cells

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• 作者：Ok-Kyoung Kwon ; Mee-Young Lee ; Ji-Eun Yuk ; Sei-Ryang Oh ; Young-Won Chin ; Hyeong-Kyu Lee ; Kyung-Seop Ahn
• 关键词：Lilium lancifolium ; Inflammation ; iNOS ; COX-2 ; TNF-alpha ; NF-κ ; B ; IL-4 ; IL-13
• 刊名：Journal of Ethnopharmacology
• 出版年：2010
• 出版时间：6 July 2010
• 年：2010
• 卷：130
• 期：1
• 页码：28-34
• 全文大小：858 K

文摘

Aim of the study

Lilium lancifolium is commonly used to treat bronchitis, pneumonia, etc. In this study, we investigated the anti-inflammatory effects of methanol extracts of the root of Lilium lancifolium (LL extracts) in LPS-stimulated Raw264.7 cells.

Material and methods

Levels of NO, PGE₂ and pro-inflammatory cytokines (IL-6 and TNF-α) in the supernatant fraction were determined using sandwich ELISA. Expression of COX-2 and iNOS, phosphorylation of MAPK subgroups (ERK and JNK), and NF-κB activation in extracts were detected via Western blot and immunocytochemistry assays.

Results

The LL extract significantly inhibited NO, PGE₂, IL-6 and TNF-α production in LPS-stimulated cells, and suppressed iNOS and COX-2 expression. A mechanism-based study showed that phosphorylation of ERK1/2 and JNK and translocation of the NF-κB p65 subunit into nuclei were inhibited by the LL extract. Furthermore, interleukin-4 and interleukin-13 production in Con A-induced splenocytes was suppressed.

Conclusion

These results indicate that anti-inflammatory effects of methanol extracts from Lilium lancifolium are due to downregulation of iNOS and COX-2 via suppression of NF-κB activation and nuclear translocation as well as blocking of ERK and JNK signaling in LPS-stimulated Raw264.7 cells.