Structural similarities of fucoidans from brown algae Silvetia babingtonii and Fucus evanescens, determined by tandem MALDI-TOF mass spectrometry

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• 作者：Stanislav D. Anastyuk ; Natalia M. Shevchenko ; Pavel S. Dmitrenok ; Tatyana N. Zvyagintseva
• 关键词：Fucoidan ; Oligosaccharides ; Autohydrolysis ; MALDI-TOFMS ; MS/MS
• 刊名：Carbohydrate Research
• 出版年：2012
• 出版时间：1 September, 2012
• 年：2012
• 卷：358
• 期：Complete
• 页码：78-81
• 全文大小：372 K

文摘

Rapid mass spectrometric investigation of oligosaccharides, obtained by autohydrolysis of fucoidans from brown algae m>Silvetia babingtoniim> and m>Fucus evanescensm> (Fucales, Phaeophyceae) has shown both similarities and differences in structural features/sulfation pattern of their fragments, obtained in the same conditions. Tandem MALDI-TOF MS of fucooligosaccharides with even DP (degree of polymerization) was close to that observed for fucoidan from m>F. evanescens.m> Slight differences in tandem mass spectra of fragments with odd DP indicated, probably, sulfation at C-3 (instead of C-2 in m>F. evanescensm>) of some (1¡ú4)-linked ¦Á-l-Fucm>pm> residues and/or the presence of short blocks, built up of (1¡ú3)-linked ¦Á-l-Fucm>pm> residues.