文摘
Temperatures for storage of crapemyrtle (Lagerstroemia spp.) pollen over time were studied using clones of two interspecific hybrids (L. ¡®Cheyenne?and L. ¡®Wichita? and five species (L. indica ¡®Catawba? L. subcostata (NA 40181), L. limii (SHL2004-1), L. speciosa (MIA 36606), and L. fauriei ¡®Kiowa?. Pollen samples were stored at room temperature (23 ¡À 1 ¡ãC), 4 ¡ãC, ?0 ¡ãC and ?0 ¡ãC. Germination of pollen samples was examined at 0, 7, 15, 45, 75, or 105 days of storage (DOS) and data were analyzed using linear models. Fresh (0 DOS) pollen of L. ¡®Cheyenne?showed the highest germination (80 % ). For all other clones, pollen germination at 0 DOS ranged from 44 % (L. speciosa) to 77 % (L. fauriei ¡®Kiowa?. Pollen of all clones lost their viability within 7 DOS at room temperature. Overall, this study indicated that Lagerstroemia pollen is best maintained over time when pollen is stored under refrigeration, with a storage temperature of ?0 ¡ãC often being preferable to 4 ¡ãC. Lowering the storage temperature to ?0 ¡ãC is generally unnecessary, but not deleterious to pollen germination. Storage of viable crapemyrtle pollen for no longer than 75-105 days is adequate for allowing breeders to efficiently execute hybridization of germplasm flowering at different times or in widely separated locations.
