Analysis of lactic acid bacteria communities and their seasonal variations in a spontaneously fermented dairy product (Malian f¨¨n¨¨) by applying a cultivation/genotype-based binary model

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• 作者：Stephan Wullschleger^a ; Christophe Lacroix^a ; Bassirou Bonfoh^b ; Aminata Sissoko-Thiam^c ; Selina Hugenschmidt^a ; Edwina Romanens^a ; Sarah Baumgartner^a ; Idrissa Traoré ; ^c ; Marcus Yaffee^a ; Christoph Jans^a ; Leo Meile^a ; ^{leo.meile@hest.ethz.ch}
• 刊名：International Dairy Journal
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：29
• 期：1
• 页码：28-35
• 全文大小：1028 K

文摘

Seasonal fluctuations of lactic acid bacteria (LAB) communities were analysed in spontaneous fermentation processes of the Malian sour milk f¨¨n¨¨ through the design of a novel flow-chart protocol for rapid typing of putative LAB. The protocol is based on cultivation on semi-selective complex agar media, followed by a binary scheme for genus- and species-specific genotypic identification. Presumptive LAB (n?=?1583) were isolated and typed from 98 samples. F¨¨n¨¨ samples displayed 10⁸?cfu?mL^?1 in all seasons. Seasonal fluctuations indicated a higher prevalence of Streptococcus spp. and Enterococcus spp. in the cold and hot season, respectively. Analysis of enterococci (n?=?596) and streptococci (n?=?454) revealed the predominance of Streptococcus infantarius subsp. infantarius over Streptococcus thermophilus. The binary culture-based protocol is a rapid tool to follow LAB species diversity and seasonal variations in any complex dairy microbial ecosystem including the cultivation of strains for subsequent characterisation.