Developmental validation of a custom panel including 273 SNPs for forensic application using Ion Torrent PGM

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• 作者：Suhua Zhang^a ; Yingnan Bian^a ; Anqi Chen^b ; Hancheng Zheng^c ; Yuzhen Gao^c ; Yiping Hou^d ; Chengtao Li^a ; ^{lichengtaohla@163.com}
• 关键词：Forensic Genetics ; Personal Genome Machine (PGM) ; Massively Parallel Sequencing (MPS) ; SNP
• 刊名：Forensic Science International: Genetics
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：27
• 期：Complete
• 页码：50-57
• 全文大小：1732 K
• 卷排序：27

文摘

A customized panel including 273 forensically relevant identity SNPs chosen from public databases as well as previously related studies was developed. The first MPS-SNP panel including SNPs covering 22 human auto-chromosomes and sex-chromosomes. Sequence data obtained had sufficiently high coverage and provided reliable and accurate SNP genotyping for most of the targets. An in-house analysis tool with automated data quality control and genotype checking of the raw data was developed. Included SNP targets are efficient for individual identification and complex paternity testing in the Chinese HAN population.