Visualization of mouse barrel cortex using ex-vivo track density imaging

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• 作者：Nyoman D. Kurniawan ; Kay L. Richards ; Zhengyi Yang ; David She ; Jeremy F.P. Ullmann ; R ; al X. Moldrich ; Sha Liu ; Javier Urriola Yaksic ; Gayeshika Leanage ; Irina Kharatishvili ; Verena Wimmer ; Fern ; o Calamante ; Graham J. Galloway ; Steven Petrou ; David C. Reutens
• 关键词：CSD ; Constrained spherical deconvolution ; DEC ; Directionally encoded color ; DTI ; Diffusion tensor imaging ; DWI ; Diffusion-weighted imaging ; FOD ; Fiber orientation distribution ; FA ; Fractional anisotropy ; HARDI ; High angular-resolution diffusion-weighted imaging ; SH ; Spherical harmonics ; stTDI ; Short-tracks track density imaging ; TDI ; Track density imaging ; VPM ; Ventral posteromedial thalamic nucleus ; PMBSF ; Posterior medial barrel sub-field
• 刊名：NeuroImage
• 出版年：15 February, 2014
• 年：2014
• 卷：87
• 期：Complete
• 页码：465-475
• 全文大小：1677 K

文摘

We describe the visualization of the barrel cortex of the primary somatosensory area (S1) of ex vivo adult mouse brain with short-tracks track density imaging (stTDI). stTDI produced much higher definition of barrel structures than conventional fractional anisotropy (FA), directionally-encoded color FA maps, spin-echo T₁- and T₂-weighted imaging and gradient echo T₁/T₂*-weighted imaging. 3D high angular resolution diffusion imaging (HARDI) data were acquired at 48 micron isotropic resolution for a (3 mm)³ block of cortex containing the barrel field and reconstructed using stTDI at 10 micron isotropic resolution. HARDI data were also acquired at 100 micron isotropic resolution to image the whole brain and reconstructed using stTDI at 20 micron isotropic resolution. The 10 micron resolution stTDI maps showed exceptionally clear delineation of barrel structures. Individual barrels could also be distinguished in the 20 micron stTDI maps but the septa separating the individual barrels appeared thicker compared to the 10 micron maps, indicating that the ability of stTDI to produce high quality structural delineation is dependent upon acquisition resolution. Close homology was observed between the barrel structure delineated using stTDI and reconstructed histological data from the same samples. stTDI also detects barrel deletions in the posterior medial barrel sub-field in mice with infraorbital nerve cuts. The results demonstrate that stTDI is a novel imaging technique that enables three-dimensional characterization of complex structures such as the barrels in S1 and provides an important complementary non-invasive imaging tool for studying synaptic connectivity, development and plasticity of the sensory system.