Polyethylenimine-immobilized core-shell nanoparticles: Synthesis, characterization, and biocompatibility test

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• 作者：Montri Ratanajanchai ; Sunhapas Soodvilai ; Nuttaporn Pimpha ; Panya Sunintaboon
• 关键词：Am-P ; amino groups per particle ; ATCC ; American type culture collection ; Cfree ; concentration of PEI in diluted supernatant ; CLSM ; confocal laser scanning microscopy ; Cm ; concentration of monomer used in the reaction ; CPEI ; concentration of PEI used in the reaction ; CQ ; camphorquinone ; DC ; degree of monomer conversion ; Dcv ; volume-average diameter of the core ; DMEM ; Dulbecco's modified Eagle medium ; DMSO ; dimethylsulfoxide ; Dn ; number-average diameter ; Dv ; volume-average diameter ; ELS ; electroph
• 刊名：Materials Science and Engineering: C
• 出版年：1 January, 2014
• 年：2014
• 卷：34
• 期：Complete
• 页码：377-383
• 全文大小：1111 K

文摘

Herein, we prepared PEI-immobilized core-shell particles possessing various types of polymer cores via a visible light-induced surfactant-free emulsion polymerization (SFEP) of three vinyl monomers: styrene (St), methyl methacrylate (MMA), and 2-hydroxyethyl methacrylate (HEMA). An effect of monomers on the polymerization and characteristics of resulting products was investigated. Monomers with high polarity can provide high monomer conversion, high percentage of grafted PEI, stable particles with uniform size distribution but less amino groups per particles. All prepared nanoparticles exhibited a core-shell nanostructure, containing PEI on the shell with hydrodynamic size around 140-230 nm. For in-vitro study in Caco-2 cells, we found that the incorporation of PEI into these core-shell nanoparticles can significantly reduce its cytotoxic effect and also be able to internalized within the cells. Accordingly, these biocompatible particles would be useful for various biomedical applications, including gene transfection and intracellular drug delivery.