A fully validated GC-TOF-MS method for the quantification of fatty acids revealed alterations in the metabolic profile of fatty acids after smoking cessation

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• 作者：Michael Goettel^a ; ^b ; Reinhard Niessner^a ; Nikola Pluym^b ; Gerhard Scherer^b ; Max Scherer^b ; ^{max.scherer@abf-lab.com}
• 关键词：CV ; coefficient of variance ; EI ; electron impact ionization ; FA ; fatty acid ; FAME ; fatty acid methyl ester ; FDA ; Food and Drug Administration of the United States of America ; FID ; flame ionization detector ; GC ; gas chromatograph ; ID ; inner diameter ; LOD ; limit of detection ; LOQ ; limit of quantification ; ME ; methyl ester ; MUFA ; mono-unsaturated fatty acid ; m/z ; mass-to-charge ratio ; MS ; mass spectrometer ; PUFA ; poly unsaturated fatty acid ; QC ; quality control ; SAFA ; saturated fatty acid ; S/N ; sig
• 刊名：Journal of Chromatography B
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：1041-1042
• 期：Complete
• 页码：141-150
• 全文大小：802 K
• 卷排序：1041

文摘

A diet-controlled clinical smoking cessation study was conducted. A GC-TOF-MS metabolic profiling method for fatty acids has been developed. A comprehensive validation of the GC-TOF-MS method according to FDA guidelines was conducted. Statistical significant alterations during smoking cessation indicate a partial recovery of the fatty acid metabolism.