Study of DNA binding and bending by Bacillus subtilis GabR, a PLP-dependent transcription factor

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• 作者：Davide Amidani^a ; Angela Tramonti^b ; ^c ; Andrea Valeria Canosa^d ; Barbara Campanini^d ; Stefano Maggi^a ; Teresa Milano^c ; Martino L. di Salvo^c ; Stefano Pascarella^c ; Roberto Contestabile^c ; Stefano Bettati^e ; ^f ; Claudio Rivetti^a ; ^{claudio.rivetti@unipr.it}
• 关键词：Transcription factor ; MocR ; GABA metabolism ; PLP enzyme ; Atomic Force Microscopy
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1861
• 期：1
• 页码：3474-3489
• 全文大小：2148 K
• 卷排序：1861

文摘

GabR binds two DNA direct repeats as a dimer and bends the cognate site by ~ 80°. Proper phasing of direct repeats is crucial for GabR binding. PLP is not required for the GabR-DNA interaction. GABA reduces GabR-DNA affinity and alters slightly the shape of the complexes. Basic residues of the AT domain affect GabR-DNA binding affinity.