Inheritance of RAPD markers in an interspecific F₁ hybrid of grape between Vitis quinquangularis and V. vinifera

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• 作者：Luo ; Sulan ; He ; Puchao ; Zheng ; Xueqin ; Zhou ; Peng
• 关键词：Vitis ; Interspecific cross ; RAPD marker ; Inheritance
• 刊名：Scientia Horticulturae
• 出版年：2002
• 出版时间：February 28, 2002
• 年：2002
• 卷：93
• 期：1
• 页码：19-28
• 全文大小：127 K

文摘

Inheritance of randomly amplified polymorphic DNA (RAPD) markers in an interspecific F₁ hybrid between Vitis quinquangularis (‘83-4-96’) and V. vinifera (‘Muscat Rose’) were studied. A preliminary screen of the F₁ plants and the parents used as the interspecific cross parent was conducted with 280 RAPD primers; of these, 39 primers were polymorphic and reproducible and produced 234 scorable loci. Genomic DNA from 60 individuals of the F₁ population was then amplified with these primers; 83.4 % of the loci segregated as expected in a Mendelian fashion. According to the segregation patterns, RAPD markers were grouped into three types: (1) normal Mendelian inheritance with segregation ratio of nearly 1:1, 3:1 or 1:0 (non-segregation); (2) deviation from Mendelian segregation ratios; (3) abnormal segregation in low frequency, including non-parental markers which were present in F₁ progenies but absent in both parents. RAPD markers presenting in both parents were the major source of non-segregation markers; markers presenting in only one parent mainly showed Mendelian segregation. There were five non-parental markers. Markers showing 1:1 and 3:1 segregation were used for constructing an RAPD molecular linkage map. The results demonstrated that RAPD markers could be used to study the genomes of the wild grapes native to China.