Fe2+ concentrations up to 16 g/L did not inhibit the studied iron-oxidisers. Al3+ concentrations of 6 g/L and below enhanced iron oxidation rates. High cadmium, sulphate, and vanadium concentrations decreased iron oxidation rates. Ammonium supplementation to bioheaps would enhance the heap bioleaching process. High inhibitory ion content increases microbial sensitivity to nitrogen deficiency.
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