Multi-center evaluation of a user-friendly lateral flow assay to determine IP-10 and CCL4 levels in blood of TB and non-TB cases in Africa

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Multi-center evaluation of a user-friendly lateral flow assay to determine IP-10 and CCL4 levels in blood of TB and non-TB cases in Africa

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作者：Paul L.A.M. Corstjens^a ; ^{P.Corstjens@LUMC.NL" class="auth_mail" title="E-mail the corresponding author} ; Elisa M. Tjon Kon Fat^a ; Claudia J. de Dood^a ; Jolien J. van der Ploeg-van Schip^b ; Kees L.M.C. Franken^b ; Novel N. Chegou^c ; Jayne S. Sutherland^d ; Rawleigh Howe^e ; Adane Mihret^e ; Desta Kassa^f ; Marieta van der Vyver^g ; Jacob Sheehama^g ; Felanji Simukonda^h ; Harriet Mayanja-Kizzaⁱ ; Tom H.M. Ottenhoff^b ; Gerhard Walzl^c ; Annemieke Geluk^b ; On behalf of the AE-TBC consortium¹
关键词：CCL4 ; chemokine C&ndash ; C motif ligand 4 ; C/E ; ESAT-6/CFP-10 ; IFN-γ ; interferon gamma ; IP-10 ; IFN-γ induced protein 10 ; LTBI ; latent TB infection ; LF ; lateral flow ; LLOD ; lower limit of detection ; NEC ; non-endemic controls ; ORD ; other respiratory diseases ; QC ; quality control ; SOP ; standard operating procedure ; TB ; tuberculosis ; UCP ; up-converting phosphor
刊名：Clinical Biochemistry
出版年：2016
出版时间：January 2016
年：2016
卷：49
期：1
页码：22-31
全文大小：1311 K

文摘

•: Evaluation of user-friendly, quantitative, lateral flow tests for chemokines, IP-10 and CCL4
•: Determination of chemokine levels in the range of 100 to 100,000 pg/mL in whole blood samples
•: Multi-country study in Africa comparing TB and cases with other respiratory disease
•: Demonstration of robustness and applicability of the low-tech UCP-LF test platform in Africa
•: Dry reagent test materials allow convenient worldwide shipping and storage at ambient temperature

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