Isolation of functionally active and highly purified neuronal mitochondria from human cortex

网站地图 | English | 公务邮箱

About the library

Background
History
Leadership
Organization

Opening Hours
Collections
Help Via Email

Electronic Information Resources

Isolation of functionally active and highly purified neuronal mitochondria from human cortex

详细信息查看全文

作者：Nicolas K. Khattar^a ; Svitlana Yablonska^a ; Sergei V. Baranov^a ; Oxana V. Baranova^a ; Eric S. Kretz^a ; Timothy M. Larkin^a ; Diane L. Carlisle^a ; R. Mark Richardson^b ; ^c ; Robert M. Friedlander^a ; ^c ; ^{friedlanderr@upmc.edu" class="auth_mail" title="E-mail the corresponding author}
关键词：Neurons ; Mitochondria ; Human cortex ; Isolation ; Percoll ; Synaptosome ; Non-synaptosomal mitochondria ; Differential centrifugation ; Purified ; Functional
刊名：Journal of Neuroscience Methods
出版年：2016
出版时间：1 April 2016
年：2016
卷：263
期：Complete
页码：1-6
全文大小：820 K

文摘

•: Combined Percoll gradient centrifugation and anti-TOM22 magnetic bead extraction is used to isolate mitochondria.
•: The mitochondria obtained are neuronal (from synaptosome disruption).
•: The mitochondria obtained have minimal cytoplasmic contaminants (plasma membrane, peroxisomes, lysosomes, synaptosomes, endoplasmic reticulum).
•: The mitochondria are functionally active based on measurements of respiration and protein import.

NGLC 2004-2010.National Geological Library of China All Rights Reserved.
Add:29 Xueyuan Rd,Haidian District,Beijing,PRC. Mail Add: 8324 mailbox 100083
For exchange or info please contact us via email.