Absolute quantification of Pru av 2 in sweet cherry fruit by liquid chromatography/tandem mass spectrometry with the use of a stable isotope-labelled peptide

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Absolute quantification of Pru av 2 in sweet cherry fruit by liquid chromatography/tandem mass spectrometry with the use of a stable isotope-labelled peptide

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作者：Katsunari Ippoushi ; ^{ippoushi@affrc.go.jp" class="auth_mail" title="E-mail the corresponding author} ; Motoe Sasanuma ; Hideaki Oike ; Masuko Kobori ; Mari Maeda-Yamamoto
关键词：Pru av 2 ; Sweet cherry ; Prunus avium L. ; Absolute quantification ; Trypsin ; Liquid chromatography/tandem mass spectrometry ; Stable isotope-labelled peptide
刊名：Food Chemistry
出版年：2016
出版时间：1 August 2016
年：2016
卷：204
期：Complete
页码：129-134
全文大小：650 K

文摘

•: Pru av 2 is a protein in sweet cherry (Prunus avium L.).
•: Pru av 2 is the principal allergen of cherry and the cause of pollen food syndrome.
•: A quantitative method for Pru av 2 was developed using an AQUA peptide and LC/MS/MS.
•: A linear relationship (r² > 0.99) was observed over the range of 2.3–600 fmol/μL.
•: Coefficients of variation measured on each of the five assay days were <14%.

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