文摘
Chinese pine (Pinus tabulaeformis carr.), endemic to China, is a conifer species with extensive and fragmented distribution in North China. In this study, the genetic diversity and structure of 20 natural populations of this species were investigated using amplified fragment length polymorphism (AFLP) markers. A total of 445 fragments were revealed with 8 pairs of primers, 379 (85.17%) of which were polymorphic. A moderate level of genetic diversity was detected at the species level (Shannon's information index I聽=聽0.356, Nei's gene diversity HE聽=聽0.271) and at the population level (I聽=聽0.219, HE聽=聽0.206). Most of genetic variation was within populations while a considerable level of genetic differentiation was detected (GST聽=聽0.352, 肖ST聽=聽0.304). The high differentiation could be attributed to the complex and fragmented habitats, and a limited gene flow among populations (Nm聽=聽0.572). The Mantel test indicated that there was significant correlation (r聽=聽0.455, P聽<聽0.001) between Nei's genetic distance and geographical distance among all the populations. The results suggested that proper countermeasures should be taken to prevent the habitat further deterioration and maintain the genetic diversity of this species.
