A novel method for measuring hydraulic conductivity at the human blood-nerve barrier in vitro

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• 作者：E. Scott Helton ; Steven Palladino ; Eroboghene E. Ubogu ; ^{eeubogu@uabmc.edu}
• 关键词：paDF ; primary adult dermal fibroblasts ; pHEndECs ; primary human endoneurial endothelial cells ; THBMECs ; SV40 large T-antigen immortalized human brain microvascular endothelial cells ; THEndECs ; SV40 large T-antigen immortalized human endoneurial endothelial cells
• 刊名：Microvascular Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：109
• 期：Complete
• 页码：1-6
• 全文大小：492 K
• 卷排序：109

文摘

Experimental apparatus and methods to measure hydraulic conductivity are described. An adaptable diffusion chamber and digital camera-based bubble tracker were used. The human blood-nerve and blood-brain barrier hydraulic conductivities were obtained. Cellular immortalization does not affect blood-nerve barrier water flux in vitro. Reliable mechanistic studies of blood-nerve barrier water flux can be performed.