Fast and sensitive in vivo studies under controlled environmental conditions to substitute long-term field trials with genetically modified plants

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• 作者：Patricia Horn^a ; André ; Schlichting^b ; ¹ ; Christel Baum^b ; Ute Hammesfahr^c ; ² ; Sö ; ren Thiele-Bruhn^c ; Peter Leinweber^b ; Inge Broer^a ; ^{inge.broer@uni-rostock.de}
• 关键词：Risk assessment ; Composite Vicia hirsuta ; Agrobacterium rhizogenes ; Bacteria-soil-plant interaction ; Rhizosphere ecology ; Root exudates ; Soil
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：10 February 2017
• 年：2017
• 卷：243
• 期：Complete
• 页码：48-60
• 全文大小：1818 K
• 卷排序：243

文摘

An in vitro assessment of root and rhizosphere effects of transgenic plants thatcan detect even minor effects and correlates them to transgene expression with less space, time and labour. Dissimilarity of rhizodeposits between transgenic and non-transgenic cultivars is reflected by soil microbial PLFA ratios. Fast detection of statistically significant transgene-specific effects with a great numbers of events cultivated in small space under semi-sterile and non-sterile conditions. Early detection of effects that can prevent superfluous developments and analyses. The system might render time consuming and expensive field tests that are less sensitive, unnecessary.