Disruption of trichomes by rapid depressurization was studied as a substrate pretreatment.

Low temperature and high pressure for the static compression increased the extract recovery.

Light microscopy images showed disruption of trichomes by rapid depressurization.

Caryophyllene oxide, β-caryophyllene and α-thujone were quantified in the selected extract.

First study of trichome disruption by rapid depressurization using response surface methodology.