Developmental Refinement of Vesicle Cycling at Schaffer Collateral Synapses

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• 作者：Tobias Rose¹ ; ³ ; ^{trose@neuro.mpg.de} ; Philipp Schoenenberger¹ ; ⁴ ; Karel Jezek² ; Thomas G. Oertner¹ ; ⁵ ; ^{thomas.oertner@zmnh.uni-hamburg.de}
• 刊名：Neuron
• 出版年：2013
• 出版时间：20 March, 2013
• 年：2013
• 卷：77
• 期：6
• 页码：1109-1121
• 全文大小：1607 K

文摘

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Summary

At synapses formed between dissociated neurons, about half of all synaptic vesicles are refractory to evoked release, forming the so-called ¡°resting pool.¡± Here, we use optical measurements of vesicular pH to study developmental changes in pool partitioning and vesicle cycling in cultured hippocampal slices. Two-photon imaging of a genetically encoded two-color release sensor (ratio-sypHy) allowed us to perform calibrated measurements at individual Schaffer collateral boutons. Mature boutons released a large fraction of their vesicles during simulated place field activity, and vesicle retrieval rates were 7-fold higher compared to immature boutons. Saturating stimulation mobilized essentially all vesicles at mature synapses. Resting pool formation and a concomitant reduction in evoked release was induced by chronic depolarization but not by acute inhibition of the protein phosphatase calcineurin. We conclude that synapses in CA1 undergo a prominent refinement of vesicle use during early postnatal development that is not recapitulated in dissociated neuronal culture.