Construction and long term preservation of clonal transgenic silkworms using a parthenogenetic strain

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Construction and long term preservation of clonal transgenic silkworms using a parthenogenetic strain

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作者：Valeriya Zabelina^a ; ¹ ; ^{vzabelina@entu.cas.cz" class="auth_mail" title="E-mail the corresponding author} ; Keiro Uchino^b ; ¹ ; ^{uchinoK@affrc.go.jp" class="auth_mail" title="E-mail the corresponding author} ; Yuji Mochida^c ; ^{mochida@ss.silk.or.jp" class="auth_mail" title="E-mail the corresponding author} ; Naoyuki Yonemura^b ; ^{yonemura@affrc.go.jp" class="auth_mail" title="E-mail the corresponding author} ; Vyacheslav Klymenko^d ; ² ; ^{stemway@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Hideki Sezutsu^b ; Toshiki Tamura^b ; ^{ttamura@affrc.go.jp" class="auth_mail" title="E-mail the corresponding author} ; Franti&scaron ; ek Sehnal^a ; ^{sehnal@bc.cas.cz" class="auth_mail" title="E-mail the corresponding author}
关键词：Silkworm ; Transgenesis ; Parthenogenesis ; Cryopreservation ; Biotechnology
刊名：Journal of Insect Physiology
出版年：2015
出版时间：October 2015
年：2015
卷：81
期：Complete
页码：28-35
全文大小：977 K

文摘

•: Ovaries developing in males yield non-diapausing eggs suitable for transgenesis.
•: Parthenogenetic host or donor females elicit parthenogenesis in implanted ovaries.
•: Transgenesis of parthenogenetic stock facilitates cloning for homozygosity.
•: Parthenogenetic female lines maintain genotype faithfully for many generations.
•: Ovary storage in liquid nitrogen allows long-term genotype conservation.

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