MicroRNA-98 plays a critical role in experimental myocarditis

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• 作者：Xiao Chen^a ; ¹ ; Shuo Dong^a ; ¹ ; Ningning Zhang^a ; Liang Chen^a ; Mao-Gang Li^b ; Ping-Chang Yang^b ; Jiangping Song^a ; ^{fwsongjiangping@hotmail.com}
• 关键词：Heart ; Myocarditis ; MicroRNA ; Interleukin-10 ; B cells
• 刊名：International Journal of Cardiology
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：229
• 期：Complete
• 页码：75-81
• 全文大小：1223 K
• 卷排序：229

文摘

Myocarditis is inflammation in the heart; its pathogenesis is to be further investigated. Activities of micro RNAs (miR) are associated with immune inflammation. This study tests a hypothesis that miR-98 is involved in the development of myocarditis.MethodsBALB/c mice were immunized with cardiac α-myosin heavy chain peptides (MyHC-α) to induce myocarditis. The effects of miR-98 on regulation of interleukin (IL)-10 were assessed by real time RT-PCR.ResultsMice immunized with MyHC-α showed myocarditis and lower frequency of IL-10+ B cells (B10 cell) in the hearts. Expression of miR-98 was higher, IL-10 was lower, in B cells isolated from the mouse hearts with myocarditis, which was negatively correlated with each other. Exposure to tumor necrosis factor-α up regulated miR-98 expression in B cells. Over-expression of miR-98 suppressed IL-10 expression in B cells. Blocking miR-98 or adoptively transplanting B10 cells attenuated experimental myocarditis.ConclusionsmiR-98 suppresses IL-10 expression in B cells in the heart, which plays an important role in myocarditis. MiR-98 may be a therapeutic target in the treatment of myocarditis.