Bone-marrow mononuclear cells reduce neurodegeneration in hippocampal CA1 layer after transient global ischemia in rats

详细信息    查看全文

• 作者：Alane Bernardo Ramos ; Andr¨¦ia Vasconcelos-dos-Santos ; Sergio Augusto Lopes de Souza ; Paulo Henrique Rosado-de-Castro ; Lea Mirian Barbosa da Fonseca ; Bianca Gutfilen ; Wagner Monteiro Cintra ; Rosalia Mendez-Otero
• 关键词：Global cerebral ischemia ; BMMCs ; Neurodegeneration ; Hippocampus
• 刊名：Brain Research
• 出版年：2013
• 出版时间：19 July, 2013
• 年：2013
• 卷：1522
• 期：Complete
• 页码：1-11
• 全文大小：5301 K

文摘

Global cerebral ischemia (GCI) results in death of the pyramidal neurons in the CA1 layer of the hippocampus. In this study we used the four-vessel occlusion (4VO) model of GCI to investigate a potential neuroprotective role of bone-marrow mononuclear cells (BMMCs) transplantation. BMMCs (3¡Á10⁷) were injected through the carotid artery, 1 or 3 days after ischemia (DAI), and the number of cells undergoing degeneration was investigated in brains at 7 DAI. A significant decrease in the number of dying cells was observed in the treated group, compared to animals treated with saline. Biodistribution of the injected cells (1 or 3 DAI) was investigated by ^99mTechnetium labeling of the BMMCs and subsequent image analysis 2 h after transplantation. In addition, the presence of CellTrace^?-labeled BMMCs was investigated in tissue sections of the hippocampal area of these transplanted animals. BMMCs treatment significantly reduced the number of FJ-C positive cells in the hippocampal CA1 layer at 7 DAI. We also observed a decrease in the number of activated microglia/macrophage (ED1-positive cells) in the BMMCs-treated group compared with the untreated group. Our data show that BMMCs are able to modulate the microglial response and reduce neurodegeneration in the CA1 layer.