A Blueprint for a Synthetic Genetic Feedback Controller to Reprogram Cell Fate

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• 作者：Domitilla Del Vecchio¹ ; ² ; ⁹ ; ^ddv@mit.edu ; Hussein Abdallah³ ; Yili Qian¹ ; James J. Collins² ; ⁴ ; ⁵ ; ⁶ ; ⁷ ; ⁸
• 关键词：feedback control ; synthetic biology ; cell fate ; reprogramming ; multistability ; gene regulatory network
• 刊名：Cell Systems
• 出版年：2017
• 出版时间：25 January 2017
• 年：2017
• 卷：4
• 期：1
• 页码：109-120.e11
• 全文大小：4714 K
• 卷排序：4

文摘

Control of TFs in a GRN is a critical aspect of directing cell fate Control via fixed overexpression relies on endogenous GRN dynamics High gain feedback overexpression control is robust to GRN dynamics Controller can be realized with a synthetic genetic circuit using siRNA technology