Oil/water nano-emulsion loaded with cobalt ferrite oxide nanocubes for photo-acoustic and magnetic resonance dual imaging in cancer: in vitro and preclinical studies

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• 作者：Raffaele Vecchione ; PhD^a ; ^b ; ¹ ; Vincenzo Quagliariello ; PhD^c ; ^d ; ¹ ; Pierangela Giustetto ; MD^e ; Dominic Calabria ; MD^b ; Ayyappan Sathya ; PhD^f ; Roberto Marotta ; PhD^f ; Martina Profeta ; MD^a ; ^b ; Simone Nitti^f ; Niccolò ; Silvestri ; MD^f ; Teresa Pellegrino ; PhD^f ; Rosario V. Iaffaioli^c ; ^d ; ^{r.iaffaioli@istitutotumori.na.it} ; Paolo Antonio Netti ; PhD^a ; ^b ; ^{paolo.netti@iit.it}
• 关键词：Nano-emulsion ; Dual-imaging ; Cubic nanoparticles ; Photo-acoustic ; MRI
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：13
• 期：1
• 页码：275-286
• 全文大小：2471 K
• 卷排序：13

文摘

Dual imaging dramatically improves detection and early diagnosis of cancer. In this work we present an oil in water (O/W) nano-emulsion stabilized with lecithin and loaded with cobalt ferrite oxide (Co0.5Fe2.5O4) nanocubes for photo-acoustic and magnetic resonance dual imaging. The nanocarrier is responsive in in vitro photo-acoustic and magnetic resonance imaging (MRI) tests. A clear and significant time-dependent accumulation in tumor tissue is shown in in vivo photo-acoustic studies on a murine melanoma xenograft model. The proposed O/W nano-emulsion exhibits also high values of r2/r1 (ranging from 45 to 85, depending on the magnetic field) suggesting a possible use as T2 weighted image contrast agents. In addition, viability and cellular uptake studies show no significant cytotoxicity on the fibroblast cell line. We also tested the O/W nano-emulsion loaded with curcumin against melanoma cancer cells demonstrating a significant cytotoxicity and thus showing possible therapeutic effects in addition to the in vivo imaging.