A universal locked nucleic acid-integrated X-shaped DNA probe design for amplified fluorescence detection of single-nucleotide variant

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• 作者：Fang Wu^a ; ¹Author Vitae ; Mei Chen^b ; ¹Author Vitae ; Jianming Lan^aAuthor Vitae ; Yaokun Xia^bAuthor Vitae ; Mengmeng Liu^bAuthor Vitae ; Wenhui He^bAuthor Vitae ; Chunyan Li^aAuthor Vitae ; Xiaosong Chen^c ; ^{chenxiaosong74@163.com}Author Vitae ; Jinghua Chen^b ; ^{cjh_huaxue@126.com}Author Vitae
• 关键词：Single-nucleotide variant ; X-shaped DNA probe ; Locked nucleic acid ; Toehold-mediated strand-displacement reaction ; β-thalassemia ; Fluorescence detection
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2017
• 出版时间：31 March 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：123-128
• 全文大小：943 K
• 卷排序：241

文摘

An enzyme-free and selective fluorescence biosensor for β-thalassemia SNV detection is proposed based on X-shaped probe. Locked nucleic acid integrated in X-shaped DNA probe improves the stability and specificity of the sensor. Signal amplification of the fluorescence biosensor lies in the toehold-mediated strand-displacement reaction assisted recycling.