Visualizing double-stranded RNA distribution and dynamics in living cells by dsRNA binding-dependent fluorescence complementation

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Visualizing double-stranded RNA distribution and dynamics in living cells by dsRNA binding-dependent fluorescence complementation

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作者：Xiaofei Cheng^a ; ^b ; Ping Deng^a ; Hongguang Cui^a ; Aiming Wang^a ; ^{aiming.wang@agr.gc.ca" class="auth_mail" title="E-mail the corresponding author}
关键词：Live-cell imaging ; Double-stranded RNA ; RNA binding protein ; Fluorescent protein ; BiFC ; Positive-sense RNA virus ; Viral replication complex ; SGS3 ; RDR6 ; D-bodies
刊名：Virology
出版年：2015
出版时间：November 2015
年：2015
卷：485
期：Complete
页码：439-451
全文大小：5660 K

文摘

•: A live-cell imaging system was developed for visualizing dsRNA in vivo.
•: It uses dsRNA binding proteins fused with two halves of a fluorescent protein.
•: Binding to a common dsRNA enables the reporter to become fluorescent.
•: The system can efficiently monitor viral RNA replication in living cells.

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