Intercellular exchanges of membrane fragments (trogocytosis) between human muscle cells and immune cells: A potential mechanism for the modulation of muscular immune responses

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• 作者：Anne Waschbisch ; Sven G. Meuth ; Alex ; er M. Herrmann ; Barbara Wrobel ; Nicholas Schwab ; Hanns Lochmü ; ller ; Heinz Wiendl
• 关键词：Inflammatory myopathy ; T cell receptor ; Costimulation ; Autoimmunity ; Trogocytosis
• 刊名：Journal of Neuroimmunology
• 出版年：2009
• 出版时间：30 April 2009
• 年：2009
• 卷：209
• 期：1-2
• 页码：131-138
• 全文大小：713 K

文摘

Trogocytosis is a cell-contact dependent intercellular transfer of membrane fragments and associated molecules. We studied trogocytosis in the interaction of T cells with human skeletal muscle cells modeling muscle–immune cell interactions under pathophysiological conditions i.e. myositis. Human myoblasts donate membrane fragments to T cells. Acquisition of muscle-derived membrane molecules depended on T-cell activation, was independent of T-cell receptor engagement, sensitive to inhibition of actin polymerization and amplified by protein kinase C activation. Single-cell patch clamping was used to demonstrate the change in membrane capacitance upon incorporation of membrane fragments in T cells. Membrane uptake was fast and temporarily, but had clear functional consequences: T cells after intimate contact with myoblasts stimulated the proliferation of autologous T cells. Our observations raise the hypothesis that trogocytosis may modulate the outcome of T–T interactions within the micromilieu of skeletal muscle.