A new strategy based on sign-on and sign-off was proposed for PG determination was developed.

PTH and MIP showed an obvious electrocatalysis and a good recognition toward PG.

The rebound PG in imprinted cavities caused the increase of PG current and the decrease of PTH current.

The sensor exhibited good selectivity and enhanced sensitivity for PG detection.

The strategy may provide a new suitable sensing tool for electroactive compounds determination.