At-line hyphenation of high-speed countercurrent chromatography with Sephadex LH-20 column chromatography for bioassay-guided separation of antioxidants from vine tea (Ampelopsis grossedentata)
At-line HSCCC-Sephadex LH-20 CC was firstly developed. Antioxidants were firstly screened by DPPH-HPLC. Seven antioxidants were purified from vine tea by the at-line configuration. Method may be extended to other natural products.
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