The Interaction Mode of the Acidic Region of the Cell Cycle Transcription Factor DP1 with TFIIH

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• 作者：Masahiko Okuda¹ ; Keigo Araki² ; Kiyoshi Ohtani² ; Yoshifumi Nishimura¹ ; ^{nisimura@tsurumi.yokohama-cu.ac.jp}
• 关键词：DBD ; DNA-binding domain ; GAL4 DBD ; DBD of GAL4 ; HSQC ; heteronuclear single quantum coherence ; LZ ; leucine zipper ; MB ; marked box ; TAD ; transactivation domain ; pRB ; retinoblastoma protein ; PH ; pleckstrin homology ; WT ; wild-type ; NOE ; nuclear Overhauser effect
• 刊名：Journal of Molecular Biology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：428
• 期：24
• 页码：4993-5006
• 全文大小：1646 K
• 卷排序：428

文摘

The function of the C-terminal region of transcription factor DP1 was unknown. The C-terminal acidic region of DP1 bound strongly to the PH domain of p62 of TFIIH. The structure of the complex formed between DP1 and p62 was solved by NMR. The transcriptional activity of E2F1-DP1 was reduced by a point mutation of DP1. The C-terminal acidic region functions as a TAD.