Reconstruction of LPS Transfer Cascade Reveals Structural Determinants within LBP, CD14, and TLR4-MD2 for Efficient LPS Recognition and Transfer

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• 作者：Je-Kyung Ryu¹ ; ² ; ⁹ ; ¹⁰ ; Soo Jin Kim³ ; ⁹ ; Sang-Hyun Rah¹ ; ² ; ⁷ ; ⁸ ; Ji In Kang³ ; Hi Eun Jung³ ; Dongsun Lee⁴ ; Heung Kyu Lee³ ; ⁴ ; Jie-Oh Lee⁵ ; Beom Seok Park⁶ ; Tae-Young Yoon¹ ; ² ; ⁷ ; ⁸ ; ^{tyyoon@yonsei.ac.kr} ; Ho Min Kim³ ; ⁴ ; ¹¹ ; ^{hm_kim@kaist.ac.kr}
• 关键词：innate immunity ; LPS recognition ; LPS transfer ; LBP ; CD14 ; TLR4/MD2 ; single-molecule fluorescence analysis ; negative-stain EM
• 刊名：Immunity
• 出版年：2017
• 出版时间：17 January 2017
• 年：2017
• 卷：46
• 期：1
• 页码：38-50
• 全文大小：7641 K
• 卷排序：46

文摘

Observation of dynamic intermediated formed during LPS transfer by TEM Reconstitution of the entire LPS transfer cascade to TLR4-MD2 on TIRF microscopy Identification of key charged residues in LBP and CD14 for dynamic LPS transfer CD14-LPS complex interact with TLR4 LRR13-LRR15 domain for LPS transfer to MD2