Purification and characterization of an antibacterial and anti-inflammatory polypeptide from Arca subcrenata

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• 作者：Yuyan Chen^a ; ¹ ; Chunlei Li^a ; ^b ; ¹ ; Jianhua Zhu^c ; ¹ ; Wangshi Xie^b ; Xianjing Hu^b ; Liyan Song^b ; ^{tsly@jnu.edu.cn} ; Jiachen Zi^a ; Rongmin Yu^a ; ^c ; ^{tyrm@jnu.edu.cn}
• 关键词：Arca subcrenata polypeptide ; Antibacterial activity ; Anti-inflammatory activity
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：177-184
• 全文大小：1423 K
• 卷排序：96

文摘

A polypeptide (PGC) was isolated from Arca subcrenata muscle. The isoelectric point of PGC was determined to be 9.76. The molecular weight was determined to be 15973.0 Da by ESI–MS/MS. N terminal amino acid sequence of PGC was shown as PSVYDAAAQLTADVKKDLRDSWKVIGGDKKGNGVA by Edman degradation. The diameter of the inhibitory ring of PGC on E.coli and S. aureus were 14.5 ± 0.44 mm and 16.5 ± 1.15 mm, respectively. The in vitro assay results indicated that the IC50 of inhibition rate for PGC on NO production was 9.60 ± 0.71 μg/mL.