Characterization of histamine Hb>2b>-like receptors in duck cerebral cortical membranes by [³H]tiotidine binding

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• 作者：Zawilska ; Jolanta B. ; Zalewska-Kaszubska ; Jadwiga ; Przybysz ; Marzena ; Nowak ; Jerzy Z.
• 关键词：Duck ; Cerebral cortex ; Tiotidine ; Histamine receptors
• 刊名：Neuroscience Letters
• 出版年：2002
• 出版时间：February 22, 2002
• 年：2002
• 卷：319
• 期：3
• 页码：149-152
• 全文大小：117 K

文摘

A selective (according to mammalian criteria) histamine (HA) Hb>2b>-receptor radioligand [³H]tiotidine ([³H]TIOT) was used to characterize HA receptors in duck cerebral cortex by an in vitro binding technique. The specific binding of [³H]TIOT to duck cerebral cortical membranes was found to be rapid, stable, saturable, reversible, and of high affinity. Saturation analysis resulted in a linear Scatchard plot suggesting binding to a single class of receptor binding sites with high affinity (Kb>d