A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA

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A novel real-time reverse transcription-polymerase chain reaction assay with partially double-stranded linear DNA probe for sensitive detection of hepatitis C viral RNA

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作者：Tianfu Liu^a ; ^b ; Zhenzhou Wan^c ; Jia Liu^b ; Lingyi Zhang^a ; Yanheng Zhou^d ; Ke Lan^b ; Yihong Hu^b ; ^{yhhu@ips.ac.cn" class="auth_mail" title="E-mail the corresponding author} ; Chiyu Zhang^b ; ^{zhangcy1999@ips.ac.cn" class="auth_mail" title="E-mail the corresponding author}
关键词：HCV ; Viral load ; Real-time RT-PCR ; Partially double-stranded linear DNA probe
刊名：Journal of Virological Methods
出版年：2016
出版时间：October 2016
年：2016
卷：236
期：Complete
页码：132-138
全文大小：1276 K

文摘

•: A novel real-time RT-PCR assay for HCV RNA detection was developed.
•: A partially double-stranded linear DNA probe was used.
•: The assay can detect various HCV genotypes.
•: The assay has a high sensitivity (78 IU/ml) and a wide linear detection range (1 × 10e2–1 × 10e¹¹ IU/ml).