Effect of chitosan molecular weight on the formation of chitosan-pullulanase soluble complexes and their application in the immobilization of pullulanase onto Fe3O4-κ-carrageenan nanoparticles
Complexation of chitosan and pullulanase was affected by chitosan molecular weight.
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Pullulanase was immobilized onto Fe3O4–κ-carrageenan nanoparticles by complexation.
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Interrelationship exists between chitosan Mw and binding characteristic.
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The high binding affinity induces more alterations of protein secondary structure.
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Changes in secondary structures impact enzymatic properties of immobilized enzyme.
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