文摘
A new analytical method has been developed and validated for the simultaneous determination of pesticides from different classes using ion chromatography-online photochemical derivatisation-fluorescence detector (IC-hv-FD). Fluorimetric detection was performed at 位ex/位em=332 nm/367 nm for imidacloprid and then detector was set at 位ex/位em=247 nm/470 nm for carbendazim. The two pesticides imidacloprid and carbendazim were successfully separated isocratically on an IonPac庐 AS11 (250 mm脳4 mm i.d; 13 碌m particle size, Dionex) anion-exchange column using 40 mM KOH with 10% (v/v) acetonitrile and pumped at a flow rate of 1.0 mL min鈭?. Under the optimized conditions, the limit of detection (LOD, S/N=3) of imidacloprid and carbendazim were 7.8 碌g L鈭? and 67 碌g L鈭?, respectively. The experimental results showed that there was good linearity with a correlation coefficient (r)鈮?.9966 over the range of 0.05-10 mg L鈭? for imidacloprid and 0.2-15 mg L鈭? for carbendazim. Good reproducibility with a relative standard deviation (RSD, n=7) less than 4.5%. Finally, the proposed method was applied with satisfactory results to the analysis of these pesticides in ground water, lake water and river water without any pre-treatment of samples. The average spiked recoveries were in the range of 90-104%.
