Enhancing capacitive DNA biosensor performance by target overhang with application on screening test of HLA-B*58:01 and HLA-B*57:01 genes

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Enhancing capacitive DNA biosensor performance by target overhang with application on screening test of HLA-B*58:01 and HLA-B*57:01 genes

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作者：Orawan Thipmanee^a ; ^b ; ^c ; Apon Numnuam^a ; ^b ; ^c ; Warakorn Limbut^a ; ^b ; ^d ; Chittanon Buranachai^a ; ^e ; Proespichaya Kanatharana^a ; ^b ; ^c ; Tirayut Vilaivan^f ; Nattiya Hirankarn^g ; ^h ; ^{Nattiya.H@chula.ac.th" class="auth_mail" title="E-mail the corresponding author} ; Panote Thavarungkul^a ; ^b ; ^e ; ^{panote.t@psu.ac.th" class="auth_mail" title="E-mail the corresponding author}
关键词：Label-free capacitive DNA biosensor ; acpcPNA probe ; Target DNA overhang ; HLA-B*58 ; 01 ; HLA-B*57 ; 01
刊名：Biosensors and Bioelectronics
出版年：2016
出版时间：15 August 2016
年：2016
卷：82
期：Complete
页码：99-104
全文大小：763 K

文摘

•: The longer the overhanging part on the electrode surface, the better the capacitive response.
•: Double stranded DNA targets were detected using a PNA probe without denaturation.
•: A capacitive system based on two acpcPNA probes differentiated between the highly similar HLA-B*58:01 and HLA-B*57:01 genes.
•: Both purified and unpurified PCR samples can be used.
•: Only 1–2 μL from the original PCR product was needed.

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