Ultrasound-assisted emulsification microextraction followed by gas chromatography-flame ionization detection for urinary methylmalonic acid determination
USAEME was developed for urinary methylmalonic acid (MMA) determination as a biomarker of vitamin B12 deficiency.
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The application of ultrasonic radiation in USAEME resulted in high extraction efficiency in a short time.
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Conversion MMA into a long-chain ester together with applying microextraction method allowed the sensitive determination of MMA by GC-FID.
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MMA was extracted by an alcoholic solvent which performs as a derivatization agent, too.
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