Introducing a fluorescence-based standard to quantify protein partitioning into membranes

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Introducing a fluorescence-based standard to quantify protein partitioning into membranes

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作者：Franziska A. Thomas¹ ; Ilaria Visco¹ ; Zden臎k Petrá ; &scaron ; ek² ; Fabian Heinemann³ ; Petra Schwille ; ^{schwille@biochem.mpg.de" class="auth_mail" title="E-mail the corresponding author}
关键词：Alexa488 ; Alexa Fluor 488 carboxylic acid ; succinimidyl ester ; BSA ; albumin from bovine serum ; DGS-NTA(Ni) ; 1 ; 2-di-(9Z-octadecenoyl)-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] nickel salt ; DOPC ; 1 ; 2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholin ; eGFP-His6 ; hexahistidine-tagged enhanced green fluorescent protein ; FCS ; fluorescence correlation spectroscopy ; GUV ; giant unilamellar vesicle ; Kd ; dissociation constant ; ; koff ; dissociation rate constant ; kon ; associat
刊名：Biochimica et Biophysica Acta (BBA)-Biomembranes
出版年：2015
出版时间：November 2015
年：2015
卷：1848
期：11
页码：2932-2941
全文大小：904 K

文摘

•: Novel assay to quantify partitioning coefficient of membrane associated proteins
•: Comparison of fluorescence correlation spectroscopy vs confocal imaging approach
•: Characterization of the affinity of His₆-tagged eGFP for GUVs with NTA(Ni) lipids
•: Confirmation that rebinding takes place for high surface densities of NTA(Ni)

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