An in vitro approach to study ameloblastin-amelogenin interactions is presented.

Intrinsic fluorescence of tryptophan and Circular Dichroism were utilized.

We report that amelogenin has ameloblastin-binding ability via its N-terminal close to Trp 25.

We report that ameloblastin has amelogenin-binding ability via a peptide encoded by exon 5.

Macromolecular co-assembly between amelogenin and ameloblastin may play important roles in enamel biomineralization.