Human lung epithelial cell A549 proteome data after treatment with titanium dioxide and carbon black

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• 作者：Ngoc Q. Vuong^a ; ^d ; Patrick Goegan^a ; Susantha Mohottalage^b ; Dalibor Breznan^a ; Marianne Ariganello^a ; Andrew Williams^c ; Fred Elisma^b ; Subramanian Karthikeyan^a ; Renaud Vincent^a ; ^d ; Premkumari Kumarathasan^b ; ^e ; ^{premkumari.kumarathasan@canada.ca" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Titanium dioxide ; Carbon black ; A549 ; Proteomics ; 2D-GE ; MALDI-TOF-TOF-MS ; Toxicity
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：8
• 期：Complete
• 页码：687-691
• 全文大小：189 K

文摘

Here, we have described the dataset relevant to the A549 cellular proteome changes after exposure to either titanium dioxide or carbon black particles as compared to the non-exposed controls, “Proteomic changes in human lung epithelial cells (A549) in response to carbon black and titanium dioxide exposures” (Vuong et al., 2016) [1]. Detailed methodologies on the separation of cellular proteins by 2D-GE and the subsequent mass spectrometry analyses using MALDI-TOF-TOF-MS are documented. Particle exposure-specific protein expression changes were measured via 2D-GE spot volume analysis. Protein identification was done by querying mass spectrometry data against SwissProt and RefSeq protein databases using Mascot search engine. Two-way ANOVA analysis data provided information on statistically significant A549 protein expression changes associated with particle exposures.