Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening

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• 作者：Joschka Willemsen¹ ; ² ; Oliver Wicht² ; Julia C. Wolanski¹ ; Nina Baur² ; Sandra Bastian¹ ; Darya A. Haas³ ; Petr Matula⁴ ; ⁵ ; Bettina Knapp⁶ ; Laurè ; ne Meyniel-Schicklin⁷ ; Chen Wang⁸ ; Ralf Bartenschlager⁹ ; Volker Lohmann⁹ ; Karl Rohr⁴ ; Holger Erfle¹⁰ ; Lars Kaderali⁶ ; ¹¹ ; Joseph Marcotrigiano⁸ ; Andreas Pichlmair³ ; Marco Binder¹ ; ² ; ¹² ; ^{m.binder@dkfz.de}
• 关键词：innate immunity ; antiviral response ; pattern recognition receptors ; signal transduction ; feedback regulation ; interferon system ; cytokines ; DAPK1 ; RIG-I ; DDX58
• 刊名：Molecular Cell
• 出版年：2017
• 出版时间：2 February 2017
• 年：2017
• 卷：65
• 期：3
• 页码：403-415.e8
• 全文大小：6202 K
• 卷排序：65

文摘

siRNA screen of antiviral RIG-I/IRF3 pathway (human kinome) was performed DAPK1 was identified as a negative-feedback regulator of the RIG-I pathway RIG-I-mediated antiviral signaling activates DAPK1 kinase activity DAPK1 inactivates RIG-I RNA sensing by direct phosphorylation of RIG-I