Gender-related difference in bloodstain RNA ratio stored under uncontrolled room conditions for 28 days

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• 作者：Baiyu Qi ; M.D.^a (Lecturer) ; Lingzhu Kong ; Ph.D.^b (Associate Professor) ; Yingqiang Lu ; M.D.^a ; ^{luyq5810@126.com} ; ^{luyingqiang5810@sina.com} (Professor)
• 关键词：Forensic science ; Bloodstain ; Age ; RT-qPCR ; RNA
• 刊名：Journal of Forensic and Legal Medicine
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：20
• 期：4
• 页码：321-325
• 全文大小：195 K

文摘

Bloodstain age is a parameter that can be used in crime scene investigations. Bloodstain age can be determined by measuring the 18S rRNA:¦Â-actin mRNA ratio by Reverse Transcription-quantitative PCR (RT-qPCR). Since this ratio is a function of time, it can be used as an estimator of bloodstain age. However, it is important to validate the technique in a variety of scenarios before it can be applied. We investigated 18S rRNA:¦Â-actin mRNA ratio in bloodstains from sixteen Chinese subjects in 28 days under uncontrolled room conditions. The ratio changed in a linear fashion. It was also found that the subjects' gender affected the relationship between time and the RNA ratio.