文摘
Shear-flow induced spore detachment was performed under well-controlled laminar flow conditions, in a specially-designed shear stress flow chamber. By comparing detachment profiles of a panel of four strains, belonging to the B. cereus group (B. cereus and B. thuringiensis) and to less related Bacillus species (B. pumilus), it was shown that the spore ability of attaching to stainless steel, probed under dynamic conditions, was mainly affected by the presence (and number) of appendages. Adhesion force between the B. cereus 98/4 strain and stainless steel was quantified at nanoscale. To this aim, detachment results were combined with a theoretical modelling, based on the balance of hydrodynamic forces and torque exerted over a simplified spore model with a spherical form. The wall shear stress, required to remove 50 % of the spores initially attached to stainless steel, was determined. On this basis, an adhesion force of 930 ± 390 pN was obtained. Real-time re-orientation of B. cereus 98/4 spores was experimentally established, by using a high-speed camera for tracking the motions of individual spores with high temporal and spatial resolution. Even though tethered to stainless steel without any detachment occurring, spores kept mobile on the substratum, probably due to the existence of discrete bonds or local clusters of anchoring sites, and tended to re-orientate in the flow direction, for minimizing hydrodynamic forces and torque exerted by fluid flow. A significant heterogeneity within the population was also observed, with the co-existence of both moving and immobile spores.
