New approach to the synthesis of oligodeoxyribonucleotides modified with phosphorothioates of predetermined sense of P-chirality

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• 作者：Barbara Nawrot ; Beata Rę ; bowska ; Katarzyna Cieś ; liń ; ska and Wojciech J. Stec
• 关键词：P-Chiral dinucleoside phosphorothioate ; Oligonucleotide phosphorothioate ; PS-DNA ; S-Protection ; Oligonucleotide synthesis
• 刊名：Tetrahedron Letters
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：46
• 期：39
• 页码：6641-6644
• 全文大小：112 K

文摘

Appropriately protected, diastereomerically pure dinucleoside phosphorothioates, obtained by the stereocontrolled oxathiaphospholane method, were S-alkylated with 2-nitrobenzyl bromide and then converted into their 3′-O-phosphoramidites. The corresponding S-protected dinucleotide building blocks were successfully used for the synthesis of oligonucleotides containing P-stereodefined phosphorothioate bonds at preselected positions.