Evaluation of fibrin-based dermal-epidermal organotypic cultures for in vitro skin corrosion and irritation testing of chemicals according to OECD TG 431 and 439

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Evaluation of fibrin-based dermal-epidermal organotypic cultures for in vitro skin corrosion and irritation testing of chemicals according to OECD TG 431 and 439

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作者：Mariana Morales^a ; David Pé ; rez^a ; Luis Correa^a ; ^b ; Luz Restrepo^a ; ^c ; ^{grupoingenieriadetejidos@udea.edu.co" class="auth_mail" title="E-mail the corresponding author}
关键词：RhE ; reconstructed human epidermis ; ORG ; organotypic skin culture ; H&E ; hematoxylin and eosin ; MTT ; 3-[4 ; 5-dimethylthiazol-2-yl]-2 ; 5-diphenyltetrazolium bromide ; BD CBA ; Becton Dickinson Cytometric Bead Array ; OD ; optical density ; RH ; relative humidity ; RT ; room temperature ; SD ; standard deviation ; OECD ; Organisation for Economic Co-operation and Development ; TG ; test guideline ; UN GHS ; United Nations Globally Harmonized System
刊名：Toxicology in Vitro
出版年：2016
出版时间：October 2016
年：2016
卷：36
期：Complete
页码：89-96
全文大小：1249 K

文摘

•: A human fibrin based dermal-epidermal organotypic skin culture (ORGs) was developed.
•: Human primary keratinocytes and fibroblasts from atypical body region were used.
•: All ORGs were built with high concentration of calcium (1.16 mM) throughout culture.
•: ORGs were useful to perform the corrosion and irritation tests under OECD Test Guides.